Genotyping and primer details


Genotyping and primer details for SALK_093974 - At4g05280
Line ID SALK_093974
Locus At4g05280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2698491 of line SALK_093974 Chr4:2698491
   
Insertion allele amplicon LBb3 - 64wa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64wa: 5'-TGTAAAGCTTGTTTGATTAAGGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2697752
   
Insertion allele amplicon R204 - BN65
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BN65: 5'-GAAGGAGAAGCGGGTATTAAGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19345059
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11