Genotyping and primer details


Genotyping and primer details for SALK_094102c - At4g13360
Line ID SALK_094102c
Locus At4g13360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7776115 of line SALK_094102c Chr4:7776115
   
Insertion allele amplicon LBb3 - 52kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52kh: 5'-CGCTTCTAAAAACAATAAACAGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7776627
   
wt amplicon (over InsPos) 18rd - 52kh
wt amplicon start Chr4:7775782
wt amplicon end Chr4:7776627
wt amplicon size 846 bp
Locus specific primer 52kh: 5'-CGCTTCTAAAAACAATAAACAGGC-3'
2nd locus specific primer 18rd: 5'-AAATCCAAAAGGACAAAAACACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11