Sequences from confirmation amplicon


Confirmation sequences for line SALK_094102c and hit At4g13360 are shown.
Line ID SALK_094102c
Locus At4g13360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7776115
Primers used for PCR 52kh - LBb3, show primer details
   
Sequence Name 35-K115696-SALK_094102C-52kh
GenBank Accession LN485221 [GenBank]
Sequencing primer gene specific primer, 52kh: 5'-CGCTTCTAAAAACAATAAACAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115696-SALK_094102C-52kh
CAAACTTAAAAAGTACTTAAACAAACAATCATCATTTTCACCAGATTATGAGCATTTTTA
GATAGCAACCAACTAGGAATGACCTTTAACTGGTGTTGTTGACCATTTCCTTATTCTTTT
CAAAAAGGTAAAACTCTAGCCAGTGTAAAGGATTTGGATATGAACATACCAACAGATCCT
CCTCCAGGGCTATGAGCTGCTATATATGAAAACCCGACATCAGGGAACAAGCCAATTCCA
TTTTCTGGCATGGCAAGAACCGTCCTCTGGAATAGAACAAAAAATATGAAAGAGAGTCAA
GAGAGTAATACCAATTATTACCTACATAATAAGTACAGAACAAGGTTTCTTTTGTTTTTG
TACCTCTGTTATCACACGGTAGCGACCATGTCCAGAAAGACCAAGACCAAATCCCATTGT
TATACCATCCATCAATGAAATATATGGCTTTTTGTACGCAGCAATTGCGGTGATACTCTG
CTGACAACTTAATAACAAATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 24-K115695-SALK_094102C-R210
GenBank Accession LN484982 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115695-SALK_094102C-R210
CTGCGTACAAAAAGCCATATATTTCATTGATGGATGGTATAACAATGGGATTTGGTCTTG
GTCTTTCTGGACATGGTCGCTACCGTGTGATAACAGAGGTACAAAAACAAAAGAAACCTT
GTTCTGTACTTATTATGTAGGTAATAATTGGTATTACTCTCTTGACTCTCTTTCATATTT
TTTGTTCTATTCCAGAGGACGGTTCTTGCCATGCCAGAAAATGGAATTGGCTTGTTCCCT
GATGTCGGGTTTTCATATATAGCAGCTCATAGCCCTGGAGGAGGATCTGTTGGTATGTTC
ATATCCAAATCCTTTACACTGGCTAGAGTTTTACCTTTTTGAAAAGAATAAGGAAATGGT
CAACAACACCAGTTAAAGGTCATTCCTAGTTGGTTGCTATCTAAAAATGCTCATAATCTG
GTGAAAATGATGATTGTTTGTTTAAGTACTTTTTAAGTTTTGTATAGAGCGGAATTCATG
TAGATGGAAGCCTGTTTATTGTTTTAAGAAGCG

Last Updated on 06.12.2018 16:11