Genotyping and primer details


Genotyping and primer details for SALK_094518 - At1g21060
Line ID SALK_094518
Locus At1g21060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7371867 of line SALK_094518 Chr1:7371867
   
Insertion allele amplicon R212 - 23au
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 23au: 5'-AAGACTGGAGCTTTTCGAGATATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7371648
   
Insertion allele amplicon R204 - SC11
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC11: 5'-TAAGCTTGTTTCAGTGTGAGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7372385
   
wt amplicon (over InsPos) SC11 - 23au
wt amplicon start Chr1:7371648
wt amplicon end Chr1:7372385
wt amplicon size 738 bp
Locus specific primer 23au: 5'-AAGACTGGAGCTTTTCGAGATATG-3'
2nd locus specific primer SC11: 5'-TAAGCTTGTTTCAGTGTGAGGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11