Sequences from confirmation amplicon


Confirmation sequences for line SALK_094518 and hit At1g21060 are shown.
Line ID SALK_094518
Locus At1g21060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7371867
Primers used for PCR SC11 - R204, show primer details
   
Sequence Name 61-K106282-SALK_094518C-R210
GenBank Accession LN485755 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K106282-SALK_094518C-R210
TCATTTGATTACTTATCTCTTTCTGCATTTAGTTTCGTTGAAATCAATTTGGGGCAAATA
ATGTGAAATGGGTTTGGTCACAGGAACTCGGAGAAGAAGCAGAATGTGATTGTTGCATCA
CCTTCAAGATTCTCTACCTACACTAAACCGGTTAGTTTCTTCTGAGCAACCAATTTCATT
TCTCTTTTAAAAAGCAAAACTTTTGTGGTTCTAAAGTCATATTTTTCATGGCAGAGTTTA
TCAATTGAGAAGAGATTACTTACTTACCAGGATTCTAATTGCAACGTTACGCCAAAGGTT
TGATCTTTATTATAGCTTTGAATGTTCATAGATTCTTTTGGTAACAAATGTACTGTTAGT
TCTAATGTTGTGTGTTTTTTGCAGTCTTCAGAAGATTTGAGGAAAGAAATTGCTTCACTT
GAGTTTGAGATTCTGCGTACAGAACAATATCTGCTCTCTCTGTATAGGACAGCTTTCGAT
GAACAAGTTTCTTCTTTTTCTCCTCACACTGAAACAAGCTT
   
Sequence Name 77-K106283-SALK_094518C-SC11
GenBank Accession LN486165 [GenBank]
Sequencing primer gene specific primer, SC11: 5'-TAAGCTTGTTTCAGTGTGAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K106283-SALK_094518C-SC11
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGACAGCAAAAAACACACAACATTAGAACTAACAGTACATTTGTTACCAAAAGAATCT
ATGAACATTCAAAGCTATAATAAAGATCAAACCTTTGGCGTAACGTTGCAATTAGAATCC
TGGTAAGTAAGTAATCTCTTCTCAATTGATAAACTCTGCCATGAAAAATATGACTTTAGA
ACCACAAAAGTTTTGCTTTTTAAAAGAGAAATGAAATTGGTTGCTCAGAAGAAACTAACC
GGTTTAGTGTAGGTAGAGAATCTTGAAGGTGATGCAACAATCACATTCTGCTTCTTCTCC
GAGTTCCTGTGACCAAACCCATTTCACATTATTTGCCCCAAATTGATTTCAACGAAACTA
AATTGCAGAAAGAGATAATGTAATCAAATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
Primers used for PCR 23au - R212, show primer details
   
Sequence Name 13-K106831-SALK_094518C-R214
GenBank Accession LN484631 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106831-SALK_094518C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGTGAGAAGATGAAGAACAATCGTGAGAAGAAGGAGAAGGAGA
ACAGAGACCCATCATTTTCTCGCACTTTCTTGTCTGTTTGTAGAGATTCATCAGAGAATA
ATATTATGAGTATGAGTGAATAATTATAATAAAATTAAGAAGACATCCCATCAAAGTATC
TTCCTTTGAGACGTCTCAAGAGCATATCTCGAAAAGCTCCAGTCT
   
Sequence Name 19-K106832-SALK_094518C-23au
GenBank Accession LN484802 [GenBank]
Sequencing primer gene specific primer, 23au: 5'-AAGACTGGAGCTTTTCGAGATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K106832-SALK_094518C-23au
CTTTGATGGGATGTCTTCTAATTTTATTATAATTATTCACTCATACTCATAATATTATTC
TCTGATGAATCTCTACAAACAGACAAGAAAGTGCGAGAAAATGATGGGTCTCTGTTCTCC
TTCTCCTTCTTCTCACGATTGTTCTTCATCTTCTCACTGATAGTTTAAACTGAAGGCGGG
AAACGACAATCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATG
ACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACT
CAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGC
GTTCAAAAGTCACCTAAGG

Last Updated on 06.12.2018 16:11