Genotyping and primer details


Genotyping and primer details for SALK_094648c - At1g01380
Line ID SALK_094648c
Locus At1g01380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:147412 of line SALK_094648c Chr1:147412
   
Insertion allele amplicon LBb3 - 01ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 01ii: 5'-GAAGCTTTGAATAAAATTTGCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:146503
   
wt amplicon (over InsPos) 15ir - 01ii
wt amplicon start Chr1:146503
wt amplicon end Chr1:147701
wt amplicon size 1199 bp
Locus specific primer 01ii: 5'-GAAGCTTTGAATAAAATTTGCGAT-3'
2nd locus specific primer 15ir: 5'-AAACCTCTCAATCTCTTCTGCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11