Genotyping and primer details


Genotyping and primer details for SALK_095004c - At1g51690
Line ID SALK_095004c
Locus At1g51690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19167610 of line SALK_095004c Chr1:19167610
   
Insertion allele amplicon LBb3 - 26ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26ht: 5'-CAATGGGTGAATAACCTACCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19168071
   
wt amplicon (over InsPos) 16rd - 26ht
wt amplicon start Chr1:19167145
wt amplicon end Chr1:19168071
wt amplicon size 927 bp
Locus specific primer 26ht: 5'-CAATGGGTGAATAACCTACCTGTT-3'
2nd locus specific primer 16rd: 5'-GTTTGTTTGCCTCTGAATGACATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11