Genotyping and primer details


Genotyping and primer details for SALK_095484c - At1g77590
Line ID SALK_095484c
Locus At1g77590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29149255 of line SALK_095484c Chr1:29149255
   
Insertion allele amplicon LBb3 - 39oh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39oh: 5'-CTGCTGTGACTAATCCTGACTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29148569
   
wt amplicon (over InsPos) 05px - 39oh
wt amplicon start Chr1:29148569
wt amplicon end Chr1:29149307
wt amplicon size 739 bp
Locus specific primer 39oh: 5'-CTGCTGTGACTAATCCTGACTCTG-3'
2nd locus specific primer 05px: 5'-CTTGGGTATTTCAAAAATGAGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11