Genotyping and primer details


Genotyping and primer details for SALK_096470c - At1g04300
Line ID SALK_096470c
Locus At1g04300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1148873 of line SALK_096470c Chr1:1148873
   
Insertion allele amplicon LBb3 - 91by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 91by: 5'-TTCCTCAGTTCAAACAACAACATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1149634
   
wt amplicon (over InsPos) 14dk - 91by
wt amplicon start Chr1:1148633
wt amplicon end Chr1:1149634
wt amplicon size 1002 bp
Locus specific primer 91by: 5'-TTCCTCAGTTCAAACAACAACATC-3'
2nd locus specific primer 14dk: 5'-GGACTTTCAGGGAAGAGTTTTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11