Genotyping and primer details


Genotyping and primer details for SALK_097767c - At3g07800
Line ID SALK_097767c
Locus At3g07800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2490047 of line SALK_097767c Chr3:2490047
   
Insertion allele amplicon LBb3 - CG71
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG71: 5'-TGATGATGGTAAAATTGTGATCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2490365
   
Insertion allele amplicon R212 - CG72
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CG72: 5'-ACAGCAGCATGAAGTAGACGTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2489456
   
wt amplicon (over InsPos) CG71 - CG72
wt amplicon start Chr3:2489456
wt amplicon end Chr3:2490365
wt amplicon size 910 bp
Locus specific primer CG72: 5'-ACAGCAGCATGAAGTAGACGTAGA-3'
2nd locus specific primer CG71: 5'-TGATGATGGTAAAATTGTGATCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11