Genotyping and primer details


Genotyping and primer details for SALK_097868c - At1g08980
Line ID SALK_097868c
Locus At1g08980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2884413 of line SALK_097868c Chr1:2884413
   
Insertion allele amplicon R204 - BL17
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL17: 5'-CCTGTAAGCCATTTCATCCATAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2884802
   
Insertion allele amplicon LBb3 - BL18
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BL18: 5'-TAGCAAGAAACTGTGAGTCTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2883837
   
wt amplicon (over InsPos) BL18 - BL17
wt amplicon start Chr1:2883837
wt amplicon end Chr1:2884802
wt amplicon size 966 bp
Locus specific primer BL17: 5'-CCTGTAAGCCATTTCATCCATAAT-3'
2nd locus specific primer BL18: 5'-TAGCAAGAAACTGTGAGTCTTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11