Genotyping and primer details


Genotyping and primer details for SALK_098531c - At5g54630
Line ID SALK_098531c
Locus At5g54630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22193938 of line SALK_098531c Chr5:22193938
   
Insertion allele amplicon LBb3 - 77xe
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 77xe: 5'-AACACTTGTTGACCCGTTTATACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22192973
   
wt amplicon (over InsPos) 33xo - 77xe
wt amplicon start Chr5:22192973
wt amplicon end Chr5:22194485
wt amplicon size 1513 bp
Locus specific primer 77xe: 5'-AACACTTGTTGACCCGTTTATACC-3'
2nd locus specific primer 33xo: 5'-GGTGAGTTGAGACATCGTAGACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11