Genotyping and primer details


Genotyping and primer details for SALK_098534c - At3g60840
Line ID SALK_098534c
Locus At3g60840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22477945 of line SALK_098534c Chr3:22477945
   
Insertion allele amplicon LBb3 - 15jg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15jg: 5'-TTCTTTCACTTGTCTCATCAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22477458
   
wt amplicon (over InsPos) 12km - 15jg
wt amplicon start Chr3:22477458
wt amplicon end Chr3:22478439
wt amplicon size 982 bp
Locus specific primer 15jg: 5'-TTCTTTCACTTGTCTCATCAAACG-3'
2nd locus specific primer 12km: 5'-CACGTAAATATTTCAGGTCCGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11