Genotyping and primer details


Genotyping and primer details for SALK_099019c - At1g55920
Line ID SALK_099019c
Locus At1g55920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20912322 of line SALK_099019c Chr1:20912322
   
Insertion allele amplicon LBb3 - 62cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62cs: 5'-GCTTGGTAGGTTTAAATTGCTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20912674
   
wt amplicon (over InsPos) 02fo - 62cs
wt amplicon start Chr1:20911961
wt amplicon end Chr1:20912674
wt amplicon size 714 bp
Locus specific primer 62cs: 5'-GCTTGGTAGGTTTAAATTGCTGAG-3'
2nd locus specific primer 02fo: 5'-GCACTAAAAACATGTGATGGCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11