Genotyping and primer details


Genotyping and primer details for SALK_099193c - At4g10230
Line ID SALK_099193c
Locus At4g10230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6366995 of line SALK_099193c Chr4:6366995
   
Insertion allele amplicon LBb3 - 39ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39ux: 5'-TAATGATAACATGAAGACACCCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6366558
   
wt amplicon (over InsPos) 72vl - 39ux
wt amplicon start Chr4:6366558
wt amplicon end Chr4:6367685
wt amplicon size 1128 bp
Locus specific primer 39ux: 5'-TAATGATAACATGAAGACACCCCC-3'
2nd locus specific primer 72vl: 5'-TTATATCTAAGGCCAAGTCCAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11