Genotyping and primer details


Genotyping and primer details for SALK_100402 - At5g24450
Line ID SALK_100402
Locus At5g24450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8352670 of line SALK_100402 Chr5:8352670
   
Insertion allele amplicon LBb3 - 21rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21rb: 5'-TTGACAATTTGAGACTCCGTGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8352070
   
wt amplicon (over InsPos) 36rz - 21rb
wt amplicon start Chr5:8352070
wt amplicon end Chr5:8353090
wt amplicon size 1021 bp
Locus specific primer 21rb: 5'-TTGACAATTTGAGACTCCGTGTAG-3'
2nd locus specific primer 36rz: 5'-TCTTGCTCGTAAATCGGATACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11