Genotyping and primer details


Genotyping and primer details for SALK_100906c - At5g08620
Line ID SALK_100906c
Locus At5g08620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2797201 of line SALK_100906c Chr5:2797201
   
Insertion allele amplicon LBb3 - 34mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34mv: 5'-CCTTAACGTCAGAGAGATCCATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2796572
   
wt amplicon (over InsPos) 08oj - 34mv
wt amplicon start Chr5:2796572
wt amplicon end Chr5:2797538
wt amplicon size 967 bp
Locus specific primer 34mv: 5'-CCTTAACGTCAGAGAGATCCATTC-3'
2nd locus specific primer 08oj: 5'-CGATAATTTCTCTTTGCAGGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11