Sequences from confirmation amplicon


Confirmation sequences for line SALK_100906c and hit At5g08620 are shown.
Line ID SALK_100906c
Locus At5g08620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2797201
Primers used for PCR 34mv - LBb3, show primer details
   
Sequence Name 24-K114624-SALK_100906C-34mv
GenBank Accession LN484979 [GenBank]
Sequencing primer gene specific primer, 34mv: 5'-CCTTAACGTCAGAGAGATCCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114624-SALK_100906C-34mv
TCGCAAGTCCAGAGTATTATCCTTGTGACATCTGATGTATCTGCTCGTGGTGTTGATTAC
CCTGATGTGTCCTTAGTCGTACAGGTAAATCATGTAGTAGTTTTCAATCATGGAATTGAA
CAGTATCTACAAAGTTGATTGTTTTTTTTTACCGAAGTTGTTGTTGTGTGTATGTTGCTT
TTGCAGATGGGATTGCCATCAGACAGGGAACAATATATACATAGACTTGGCAGAACTGGG
CGGAAAGGTAAGGAAGGAGAAGGTGTACTATTGTTGGCACCGTGGGAAGAGTACTTTCTA
TCTTCTGTTAAGGACTTGCCCATCACAAAGTCTTCTTTACCGCCAATAGACCATGAGGCT
GTGAAAAAGGTAAGATTTTTCAAATACTACTACTGTAAACTTAGCCTGAATTTGTGACTC
TATTGAACTTAGCAAGCCTTTCGTAAATTCTATTCGCTAGCAAGTAGCAAACCAGAACAA
CCAGAATGTTATATAAACTGTATTGGAATATGTATGCAAAGGAATAAGATTTTCACATCT
CACGAAACTAACCTCTTTTACGTTAGACAAATTGACGCTNAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 08-K114623-SALK_100906C-R210
GenBank Accession LN484499 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114623-SALK_100906C-R210
TTTGTCTACGTAAAGAGGTTAGTTTCGTGAGATGTGAAAATCTTATTCCTTTGCATACAT
ATTCCAATACAGTTTATATAACATTCTGGTTGTTCTGGTTTGCTACTTGCTAGCGAATAG
AATTTACGAAAGGCTTGCTAAGTTCAATAGAGTCACAAATTCAGGCTAAGTTTACAGTAG
TAGTATTTGAAAAATCTTACCTTTTTCACAGCCTCATGGTCTATTGGCGGTAAAGAAGAC
TTTGTGATGGGCAAGTCCTTAACAGAAGATAGAAAGTACTCTTCCCACGGTGCCAACAAT
AGTACACCTTCTCCTTCCTTACCTTTCCGCCCAGTTCTGCCAAGTCTATGTATATATTGT
TCCCTGTCTGATGGCAATCCCATCTGCAAAAGCAACATACACACAACAACAACTTCGGTA
AAAAAAAACAATCAACTTTGTAGATACTGTTCAATTCCATGATTGAAAACTACTACATGA
TTTACCTGTACGACTAAGGACACATCAGGGTAATCAACACCACGAGCAGATACATCAGAT
GTCACAAGGATAATACTCTTGGACTTGCGGAACTCATCAGAAACTCTGGTTCTGTAACTC
TGCGGTTTTCTAGAANGGATCTCT

Last Updated on 06.12.2018 16:11