Genotyping and primer details


Genotyping and primer details for SALK_101706c - At1g32260
Line ID SALK_101706c
Locus At1g32260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11641924 of line SALK_101706c Chr1:11641924
   
Insertion allele amplicon LBb3 - 22rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22rb: 5'-TCAAGTCTCATTGATTGGTTTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11641481
   
wt amplicon (over InsPos) 35rz - 22rb
wt amplicon start Chr1:11641481
wt amplicon end Chr1:11642376
wt amplicon size 896 bp
Locus specific primer 22rb: 5'-TCAAGTCTCATTGATTGGTTTGAG-3'
2nd locus specific primer 35rz: 5'-TCAAACGAACGGTTTGATTTAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11