Genotyping and primer details


Genotyping and primer details for SALK_101982c - At1g45000
Line ID SALK_101982c
Locus At1g45000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17009629 of line SALK_101982c Chr1:17009629
   
Insertion allele amplicon LBb3 - 93by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 93by: 5'-AAACAACAGGATCAACCTACCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17010051
   
wt amplicon (over InsPos) 12dt - 93by
wt amplicon start Chr1:17009129
wt amplicon end Chr1:17010051
wt amplicon size 923 bp
Locus specific primer 93by: 5'-AAACAACAGGATCAACCTACCAAC-3'
2nd locus specific primer 12dt: 5'-CTTAACCCATTCCTCAAGAAAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11