Genotyping and primer details


Genotyping and primer details for SALK_102687 - At3g11020
Line ID SALK_102687
Locus At3g11020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3456517 of line SALK_102687 Chr3:3456517
   
Insertion allele amplicon LBb3 - 04au
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04au: 5'-GGGTAAAGGAGGACCAGATAATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3456206
   
Insertion allele amplicon R204 - AD04
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AD04: 5'-CTCATTAGGATCCCAAGAAGTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3456839
   
wt amplicon (over InsPos) 04au - AD04
wt amplicon start Chr3:3456206
wt amplicon end Chr3:3456839
wt amplicon size 634 bp
Locus specific primer AD04: 5'-CTCATTAGGATCCCAAGAAGTCTG-3'
2nd locus specific primer 04au: 5'-GGGTAAAGGAGGACCAGATAATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11