Genotyping and primer details


Genotyping and primer details for SALK_103627c - At1g79170
Line ID SALK_103627c
Locus At1g79170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29782441 of line SALK_103627c Chr1:29782441
   
Insertion allele amplicon LBb3 - 27ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27ty: 5'-ACGGACGTTTTCATAACACCTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29781913
   
wt amplicon (over InsPos) 61vr - 27ty
wt amplicon start Chr1:29781913
wt amplicon end Chr1:29783059
wt amplicon size 1147 bp
Locus specific primer 27ty: 5'-ACGGACGTTTTCATAACACCTAAC-3'
2nd locus specific primer 61vr: 5'-CAAAAATAAAACATCGAGAAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11