Genotyping and primer details


Genotyping and primer details for SALK_103699c - At1g11840
Line ID SALK_103699c
Locus At1g11840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3995933 of line SALK_103699c Chr1:3995933
   
Insertion allele amplicon LBb3 - 46wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46wu: 5'-AAAAAGCCGTGTATCTCTTCGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3996588
   
wt amplicon (over InsPos) 15xf - 46wu
wt amplicon start Chr1:3995571
wt amplicon end Chr1:3996588
wt amplicon size 1018 bp
Locus specific primer 46wu: 5'-AAAAAGCCGTGTATCTCTTCGTTA-3'
2nd locus specific primer 15xf: 5'-ATTTTATTCTAGCGATCCGGTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11