Sequences from confirmation amplicon


Confirmation sequences for line SALK_103699c and hit At1g11840 are shown.
Line ID SALK_103699c
Locus At1g11840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3995933
Primers used for PCR 46wu - LBb3, show primer details
   
Sequence Name 42-K118859-SALK_103699C-46wu
GenBank Accession LN485343 [GenBank]
Sequencing primer gene specific primer, 46wu: 5'-AAAAAGCCGTGTATCTCTTCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118859-SALK_103699C-46wu
ACCTAAAGCTCAAAGTAATAGGACTTACATCTTGAGTTGAAATGGCAAAATGCCCAAAGC
CAGTTCCAATATCATATGAGCTAACACCATAGTCTGCGAAACACAAAACATTAGAACAAA
GTAGTTAACTTGTGAAAAAGCGTATAACAGGAGAGGGCGAGTAGAGTGCATACTATATGT
GAGCTCCACAACAAAGTTAGATGTTTCAGGTCCAAAACCAAGAAAAGCATTAGAATACTT
CTCCTCAGGGATATCTCTTTTCCTCAACAGCTTCATACCAAAGACCTCAGTGTAAAACCT
GGTGAGACAAATAAAGAATATCTTAAAATGTAAAATAACTCCTGTTACAGAAAGATAAAG
AATAAGGTATTAATGAAAAAGAAAAAGACATACTCAATGGTGCGATCAAGATCACCAACG
CGGTAGACAACATGGAGAAAACGACGGTTATCCTTCTTAGGCCATTCCAACAAATCAGAA
GCCTCAGCCATGTTTCTAGAAAGAAGAAAACTTGATTCAGCTGCTCTCATTGAAACAAAA
TGTACATTACAAATCGAGATAAAACATTGACACAGCCTCAACATAGAGGCGGAAGCAATC
TCAATGTAAACAAATTGACGCACCATCAAATCAGGATATATTGTGGTGTAAACAAATTGA
CGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTAGATGGTGGTT
   
Sequence Name 18-K118858-SALK_103699C-R210
GenBank Accession LN484799 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118858-SALK_103699C-R210
GTCATTTGTTTACCCACAATATATCCTGATTTGATGGTGCGTCAATTTGTTTACATTGAG
ATTGCTTCCGCCTCTATGTTGAGGCTGTGTCAATGTTTTATCTCGATTTGTAATGTACAT
TTTGTTTCAATGAGAGCAGCTGAATCAAGTTTTCTTCTTTCTAGAAACATGGCTGAGGCT
TCTGATTTGTTGGAATGGCCTAAGAAGGATAACCGTCGTTTTCTCCATGTTGTCTACCGC
GTTGGTGATCTTGATCGCACCATTGAGTATGTCTTTTTCTTTTTCATTAATACCTTATTC
TTTATCTTTCTGTAACAGGAGTTATTTTACATTTTAAGATATTCTTTATTTGTCTCACCA
GGTTTTACACTGAGGTCTTTGGTATGAAGCTGTTGAGGAAAAGAGATATCCCTGAGGAGA
AGTATTCTAATGCTTTTCTTGGTTTTGGACCTGAAACATCTAACTTTGTTGTGGAGCTCA
CATATAGTATGCACTCTACTCGCCCTCTCCTGTTATACGCTTTTTCACAAGTTAACTACT
TTGTTCTAATGTTTTGTGTTTCGCAGACTATGGTGTTAGCTCATATGATATTGGAACTGG
CTTTGGGCATTTTGCCATTTCAACTCAAGATGTAAGTCCTATTACTTTGAGCTTTAGGTA
TCAAGAATCAAAATATGCATGTGATCCTTAACGAAG

Last Updated on 06.12.2018 16:11