Genotyping and primer details


Genotyping and primer details for SALK_104250 - At1g05750
Line ID SALK_104250
Locus At1g05750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1721742 of line SALK_104250 Chr1:1721742
   
Insertion allele amplicon LBb3 - 25gm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25gm: 5'-TGATTTGGATTTACTTGTGCTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1721365
   
wt amplicon (over InsPos) 17hs - 25gm
wt amplicon start Chr1:1721365
wt amplicon end Chr1:1722247
wt amplicon size 883 bp
Locus specific primer 25gm: 5'-TGATTTGGATTTACTTGTGCTGAG-3'
2nd locus specific primer 17hs: 5'-CTCACATTGTTCTTGAAATCCTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11