Genotyping and primer details


Genotyping and primer details for SALK_105118c - At2g04350
Line ID SALK_105118c
Locus At2g04350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1518458 of line SALK_105118c Chr2:1518458
   
Insertion allele amplicon LBb3 - 42kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42kh: 5'-CGTAATTGTGAAAAACCTGAGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1518971
   
wt amplicon (over InsPos) 04mu - 42kh
wt amplicon start Chr2:1517909
wt amplicon end Chr2:1518971
wt amplicon size 1063 bp
Locus specific primer 42kh: 5'-CGTAATTGTGAAAAACCTGAGACA-3'
2nd locus specific primer 04mu: 5'-TCAATATCTGCATGGGGTAATGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11