Genotyping and primer details


Genotyping and primer details for SALK_105170c - At1g30650
Line ID SALK_105170c
Locus At1g30650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10869008 of line SALK_105170c Chr1:10869008
   
Insertion allele amplicon LBb3 - 42dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42dr: 5'-ATCACAAATTTCCCAAGAAAACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10868429
   
wt amplicon (over InsPos) 06ep - 42dr
wt amplicon start Chr1:10868429
wt amplicon end Chr1:10869223
wt amplicon size 795 bp
Locus specific primer 42dr: 5'-ATCACAAATTTCCCAAGAAAACAC-3'
2nd locus specific primer 06ep: 5'-TTTGATCCAAGATCGAGAAAAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11