Sequences from confirmation amplicon


Confirmation sequences for line SALK_105170c and hit At1g30650 are shown.
Line ID SALK_105170c
Locus At1g30650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10869008
Primers used for PCR 42dr - LBb3, show primer details
   
Sequence Name 74-K110311-SALK_105170C-42dr
GenBank Accession LN486101 [GenBank]
Sequencing primer gene specific primer, 42dr: 5'-ATCACAAATTTCCCAAGAAAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K110311-SALK_105170C-42dr
AGAAGATAGTCTCTCTAATTCTCTCTCTCCCTCGGATTCGTTAGATGTGATCATTTTTTC
TTGATTTTTGATCAGATTCTTGCGTATAGCTGTATATATACACCAAGATACACTCATCAT
CGTCATATATAGATTATGTGCAGCGTCTCTGAGCTTCTTGACATGGAAAACTTCCAAGGA
GACTTAACCGACGTCGTACGAGGAATCGGAGGCCACGTGTTATCACCGGAGACTCCTCCC
TCGAACATCTGGCCTCTTCCTCTGTCACATCCAACACCATCACCGTCAGATCTTAACATA
AACCCCTTCGGAGATCCCTTTGTGAGCATGGACGATCCACTCCTCCAAGAACTAAACTCC
ATCACAAACTCCGGCTATTTCTCCACCGTAGGAGATAACAACAACAACATTCACAACAAC
AATGGTTTCTTGGTTCCAAAGGTATTTGAGGAGGATCATATAAAGAGTCAATGTAGTATC
TTCCCAAGAATCCGGATCTCGCATAGTAACATCATCCACGATTCTTCTCCGTATATTGTG
GTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACT
GGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGG
CCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGT
TTGATGGTGGTTCCGAAATCGGCAAA
   
Sequence Name 64-K110310-SALK_105170C-R210
GenBank Accession LN485842 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K110310-SALK_105170C-R210
GCGTCATTTGTTTACCCACAATATACGGAGAAGAATCGTGGATGATGTTACTATGCGAGA
TCCGGATTCTTGGGAAGATACTACATTGACTCTTTATATGATCCTCCTCAAATACCTTTG
GAACCAAGAAACCATTGTTGTTGTGAATGTTGTTGTTGTTATCTCCTACGGTGGAGAAAT
AGCCGGAGTTTGTGATGGAGTTTAGTTCTTGGAGGAGTGGATCGTCCATGCTCACAAAGG
GATCTCCGAAGGGGTTTATGTTAAGATCTGACGGTGATGGTGTTGGATGTGACAGAGGAA
GAGGCCAGATGTTCGAGGGAGGAGTCTCCGGTGATAACACGTGGCCTCCGATTCCTCGTA
CGACGTCGGTTAAGTCTCCTTGGAAGTTTTCCATGTCAAGAAGCTCAGAGACGCTGCACA
TAATCTATATATGACGATGATGAGTGTATCTTGGTGTATATATACAGCTATACGCAAGAA
TCTGATCAAAAATCAAGAAAAAATGATCACATCTAACGAATCCGAGGGAGAGAGAGAATT
AGATGAGACTATCTTTCTATATATAGCATCATATATGTGAGTGTTTTCTTGGGAAATTTG
TGA

Last Updated on 06.12.2018 16:11