Genotyping and primer details

Genotyping and primer details for SALK_105822c - At5g38110
Line ID SALK_105822c
Locus At5g38110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15209405 of line SALK_105822c Chr5:15209405
Insertion allele amplicon LBb3 - 35mv
Locus specific primer 35mv: 5'-CTCCATTTACAACAGAATCAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15209942
wt amplicon (over InsPos) 18oj - 35mv
wt amplicon start Chr5:15208844
wt amplicon end Chr5:15209942
wt amplicon size 1099 bp
Locus specific primer 35mv: 5'-CTCCATTTACAACAGAATCAGCAA-3'
2nd locus specific primer 18oj: 5'-TGAAGAATGAAGAACCTGTGATTG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11