Genotyping and primer details


Genotyping and primer details for SALK_106046c - At5g09740
Line ID SALK_106046c
Locus At5g09740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3023905 of line SALK_106046c Chr5:3023905
   
Insertion allele amplicon LBb3 - 27eb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27eb: 5'-TGGGTACTATCTTCAGCAATGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3023377
   
Insertion allele amplicon LBb3 - CE74
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE74: 5'-AAAGGTGGAAATTTAGTCAAGGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3024329
   
wt amplicon (over InsPos) CE73 - CE74
wt amplicon start Chr5:3023315
wt amplicon end Chr5:3024329
wt amplicon size 1015 bp
Locus specific primer CE74: 5'-AAAGGTGGAAATTTAGTCAAGGGT-3'
2nd locus specific primer CE73: 5'-TTTAATGAACCGTGAATGTGGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11