Genotyping and primer details


Genotyping and primer details for SALK_106192 - At2g32380
Line ID SALK_106192
Locus At2g32380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13746939 of line SALK_106192 Chr2:13746939
   
Insertion allele amplicon LBb3 - 32jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32jd: 5'-AATCTCGCTGTTTCTGATCAATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13747391
   
wt amplicon (over InsPos) 18km - 32jd
wt amplicon start Chr2:13746589
wt amplicon end Chr2:13747391
wt amplicon size 803 bp
Locus specific primer 32jd: 5'-AATCTCGCTGTTTCTGATCAATTT-3'
2nd locus specific primer 18km: 5'-AGACCTTTTGGTGAAGTGAACTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11