Genotyping and primer details


Genotyping and primer details for SALK_106536c - At1g19120
Line ID SALK_106536c
Locus At1g19120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6608436 of line SALK_106536c Chr1:6608436
   
Insertion allele amplicon LBb3 - 39cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39cv: 5'-CATATGAGCTGGAAGCTCTTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6608904
   
wt amplicon (over InsPos) 04gc - 39cv
wt amplicon start Chr1:6607967
wt amplicon end Chr1:6608904
wt amplicon size 938 bp
Locus specific primer 39cv: 5'-CATATGAGCTGGAAGCTCTTCTTT-3'
2nd locus specific primer 04gc: 5'-AAAACCCAAATGCAAAATAAAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11