Genotyping and primer details


Genotyping and primer details for SALK_106556c - At1g09540
Line ID SALK_106556c
Locus At1g09540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3087536 of line SALK_106556c Chr1:3087536
   
Insertion allele amplicon LBb3 - 24md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 24md: 5'-GAGTTTTAGCGACAAAGACAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3086987
   
wt amplicon (over InsPos) 02kn - 24md
wt amplicon start Chr1:3086987
wt amplicon end Chr1:3087855
wt amplicon size 868 bp
Locus specific primer 24md: 5'-GAGTTTTAGCGACAAAGACAAACC-3'
2nd locus specific primer 02kn: 5'-CTTTGACGTATAAAACACGCTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11