Genotyping and primer details


Genotyping and primer details for SALK_106809 - At3g48750
Line ID SALK_106809
Locus At3g48750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18073376 of line SALK_106809 Chr3:18073376
   
Insertion allele amplicon LBb3 - 94ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 94ns: 5'-TGTTCATATGTTCCCGTTATGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18073000
   
wt amplicon (over InsPos) 02oy - 94ns
wt amplicon start Chr3:18073000
wt amplicon end Chr3:18073925
wt amplicon size 926 bp
Locus specific primer 94ns: 5'-TGTTCATATGTTCCCGTTATGAAA-3'
2nd locus specific primer 02oy: 5'-CATCACAATCAAGCATACAGGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11