Sequences from confirmation amplicon


Confirmation sequences for line SALK_106809 and hit At3g48750 are shown.
Line ID SALK_106809
Locus At3g48750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18073376
Primers used for PCR 94ns - LBb3, show primer details
   
Sequence Name 32-K114796-SALK_106809C-94ns
GenBank Accession LN485170 [GenBank]
Sequencing primer gene specific primer, 94ns: 5'-TGTTCATATGTTCCCGTTATGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114796-SALK_106809C-94ns
TGTAAATAAGTATTATACCACTTATGTGTCTGATAAATGTGATGAAAAGGACTAAGCCAT
TTCACATAGACGGTTGAAGTAAGCTAGTTCAAGATACTGTAAAATATTTCAATGCTACAT
CCAGATTTCAGATCTCTTCCTGGTTATTCACACAATTTTTGCATATTGTTCCTTTAGGTT
GTTACTCTCTGGTACCGAGCACCAGAGATACTCCTAGGATCTCATCATTACTCTACACCT
GTTGATATTTGGTCTGTGGGGTGCATATTTGCTGAGATGATCAGCCAAAAGCCCTTATTT
CCTGGAGACTCCGAGATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCA
   
Sequence Name 29-K114795-SALK_106809C-R210
GenBank Accession LN485111 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114795-SALK_106809C-R210
CACAATCTCGGAGTCTCCAGGAAATAAGGGCTTTTGGCTGATCATCTCAGCAAATATGCA
CCCCACAGACCAAATATCAACAGGTGTAGAGTAATGATGAGATCCTAGGAGTATCTCTGG
TGCTCGGTACCAGAGAGTAACAACCTAAAGGAACAATATGCAAAAATTGTGTGAATAACC
AGGAAGAGATCTGAAATCTGGATGTAGCATTGAAATATTTTACAGTATCTTGAACTAGCT
TACTTCAACCGTCTATGTGAAATGGCTTAGTCCTTTTCATCACATTTATCAGACACATAA
GTGGTATAATACTTAATTTACATTACACTCATAAGGAATGCAGCCAGCCAAAAGATTTCA
TAACGGG

Last Updated on 06.12.2018 16:11