Genotyping and primer details

Genotyping and primer details for SALK_107912c - At3g12540
Line ID SALK_107912c
Locus At3g12540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3976466 of line SALK_107912c Chr3:3976466
Insertion allele amplicon LBb3 - 49et
Locus specific primer 49et: 5'-AACTTACTGCAGGTGAAGACCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3976838
wt amplicon (over InsPos) 03go - 49et
wt amplicon start Chr3:3975939
wt amplicon end Chr3:3976838
wt amplicon size 900 bp
Locus specific primer 49et: 5'-AACTTACTGCAGGTGAAGACCAAT-3'
2nd locus specific primer 03go: 5'-GAAGGGTCAAAAACTGTTTCAAAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11