Sequences from confirmation amplicon


Confirmation sequences for line SALK_107912c and hit At3g12540 are shown.
Line ID SALK_107912c
Locus At3g12540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3976466
Primers used for PCR 49et - LBb3, show primer details
   
Sequence Name 85-K111509-SALK_107912C-49et
GenBank Accession LN486285 [GenBank]
Sequencing primer gene specific primer, 49et: 5'-AACTTACTGCAGGTGAAGACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K111509-SALK_107912C-49et
TTTGGCTCTCGCATCTGAATCCGTAGCGTGTCTGTATTCTCACTTCGCTTTCACCGGCTG
AAACCTTTTTATTCGGATAAAAGAAACATTTTAAAGATCAGTTATTACAACTTCACAGGA
TGATATATATGAGTACTTACAGATTCCCTAGGTTCGCATGGACTCTGCAAGATGGATCCT
TCGATATCTAGAGCAGACAGTTGTGTACCTCCCACGTCAATTGTTGCCTACAACACAAGT
CAATTTTGTTCATTAACTTAAGTATGCGAAGTTTAAAGATGTTTCTTGTTTTGTATTAGT
TACCATTTTGAGAATGGTCAGCTTGTGACCTTGCGGACCCTTACGTCATTGTGGTGTAAA
CAAATTGACGCTTAATAACAAATTGTGGTTTAAACTGATTTGTCTAAACTATCATTTCAT
GGTCAGTTATTGTTTACACCACACCACAATAACTTTGTTTGTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 71-K111508-SALK_107912C-R210
GenBank Accession LN486029 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K111508-SALK_107912C-R210
AACAAGTTATTGTGGTGTGGTGTAAACATAACTGACCATGAAATGATAGTTTAGACAAAT
CAGTTTAAACCACAATTTGTTATTAAGCGTCAATTTGTTACACCACAATGACGTAAGGGT
CCGCAAGGTCACAAGCTGACCATTCTCAAAATGGTAACTAATACAAAACAAGAAACATCT
TTAAACTTCGCATACTTAAGTTAATGAACAAAATTGACTTGTGTTGTAGGCAACAATTGA
CGTGGGAGGTACACAACTGTCTGCTCTAGATATCGAAGGATCCATCTTGCAGAGTCCATG
CGAACCTAGGGAATCTGTAAGTACTCATATATATCATCCTGTGAAGTTGTAATAACTGAT
CTTTAAAATGTTTCTTTTATCCGAATAAAAAGGTTTCAGCCGGTGAAAGCGAAGTGAGAA
TACAGACACGCTACGGATTCAGATGCGTAGAGCCAAACCTAATGTTCGTGCTTTGTCGTG
GAGATTGGTCTTCACCTGCAGTAAGT

Last Updated on 06.12.2018 16:11