Genotyping and primer details


Genotyping and primer details for SALK_107941c - At2g45470
Line ID SALK_107941c
Locus At2g45470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18743273 of line SALK_107941c Chr2:18743273
   
Insertion allele amplicon LBb3 - 27lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27lu: 5'-CACAACTCTCTCCACCACACTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18743742
   
wt amplicon (over InsPos) 13mu - 27lu
wt amplicon start Chr2:18742675
wt amplicon end Chr2:18743742
wt amplicon size 1068 bp
Locus specific primer 27lu: 5'-CACAACTCTCTCCACCACACTTTA-3'
2nd locus specific primer 13mu: 5'-CCTCACTCACTAATCCCATGTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11