Genotyping and primer details


Genotyping and primer details for SALK_108014c - At5g39900
Line ID SALK_108014c
Locus At5g39900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15977538 of line SALK_108014c Chr5:15977538
   
Insertion allele amplicon LBb3 - 04kw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04kw: 5'-AAGATGTGCTTCTTGTGTCTGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15978125
   
wt amplicon (over InsPos) 05ke - 04kw
wt amplicon start Chr5:15977116
wt amplicon end Chr5:15978125
wt amplicon size 1009 bp
Locus specific primer 04kw: 5'-AAGATGTGCTTCTTGTGTCTGCTA-3'
2nd locus specific primer 05ke: 5'-CCTGATGTTATGCTTTTCAACTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11