Sequences from confirmation amplicon


Confirmation sequences for line SALK_108014c and hit At5g39900 are shown.
Line ID SALK_108014c
Locus At5g39900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15977538
Primers used for PCR 04kw - LBb3, show primer details
   
Sequence Name 64-K114145-SALK_108014C-R210
GenBank Accession LN485851 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K114145-SALK_108014C-R210
TTTGTTTACCCACAATATATTGTCCACATCTCACTGATACACTTGCATCGTTGCATGTCA
GTTTCTCCATGGCATGACCAAGTGCTTCGAAATCAGATCCATCAGCAGGATAAACGCCTG
AAAACACCATATGCCTCACCGGCTTGAAACCTAGGTTCCACAAGTTAAACTCAAGTCAGC
GAGCTTTAAGAAGCTTACTGGAGTTGCACAAGACACAAAAAATAAATACCTGGAAGAGGC
TCAACAGTGGTTTTCGTTCTATAGATAGTGTCTCCAATACGTGCTTCTTTCGTGGTTCGC
ATACCGGTTACTATATAACCAACTTGTCCAGTAAGAAGCATTCCTGTAGAAGTAAGCTCA
GGATGCATAATCCCGACATCTAACACTTCATAAGACTGACCCGACGCAGCAAACGAAACC
TTATCTCCTTTACTCAACATTCCATCAACAACAGAAACATAGCANATGACACCTTTGTAC
TCATTAAAAAAAGAATCAAACAAAAGCATCCGTAGAGGCGATTCACTAATCCCAGGAGGA
GGAGGTATCCGTTCTATAACCGCAGGAAGAACATGCTCAAGACCCAAACCGGTNTTAGCA
GACACAAGAG
   
Sequence Name 75-K114146-SALK_108014C-04kw
GenBank Accession LN486133 [GenBank]
Sequencing primer gene specific primer, 04kw: 5'-AAGATGTGCTTCTTGTGTCTGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K114146-SALK_108014C-04kw
AACGGATACCTCCTCCTCCTGGGATTAGTGAATCGCCTCTACGGATGCTTTTGTTTGATT
CTTTTTTTAATGAGTACAAAGGTGTCATTTGCTATGTTTCTGTTGTTGATGGAATGTTGA
GTAAAGGAGATAAGGTTTCGTTTGCTGCGTCGGGTCAGTCTTATGAAGTGTTAGATGTCG
GGATTATGCATCCTGAGCTTACTTCTACAGGAATGCTTCTTACTGGACAAGTTGGTTATA
TAGTAACCGGTATGCGAACCACGAAAGAAGCACGTATTGGAGACACTATCTATAGAACGA
AAACCACTGTTGAGCCTCTTCCAGGTATTTATTTTTTGTGTCTTGTGCAACTCCAGTAAG
CTTCTTAAAGCTCGCTGACTTGAGTTTAACTTGTGGAACCTAGGTTTCAAGCCGGTGAGG
CATATGGTGTTTTCAGGCGTTTATCCTGCTGATGGATCTGATTTCGAAGCACTTGGTCAT
GCCATGGAGAAACTGACATGCAACGATGCANGTGTATCAGTGAGATGTGGACAATATATT
GTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTAGTTC

Last Updated on 06.12.2018 16:11