Genotyping and primer details


Genotyping and primer details for SALK_108151c - At1g79400
Line ID SALK_108151c
Locus At1g79400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29866556 of line SALK_108151c Chr1:29866556
   
Insertion allele amplicon LBb3 - 94by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 94by: 5'-TTGTAGTCTTCCCATCGATTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29867021
   
wt amplicon (over InsPos) 23dz - 94by
wt amplicon start Chr1:29866095
wt amplicon end Chr1:29867021
wt amplicon size 927 bp
Locus specific primer 94by: 5'-TTGTAGTCTTCCCATCGATTCTCT-3'
2nd locus specific primer 23dz: 5'-CATAGCTGGGAAATTTATTGGTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11