Genotyping and primer details


Genotyping and primer details for SALK_109389 - At1g08830
Line ID SALK_109389
Locus At1g08830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2828196 of line SALK_109389 Chr1:2828196
   
Insertion allele amplicon LBb3 - 20ce
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20ce: 5'-CCTCCTGAAATGTAAAACACCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2828756
   
wt amplicon (over InsPos) 20dn - 20ce
wt amplicon start Chr1:2827828
wt amplicon end Chr1:2828756
wt amplicon size 929 bp
Locus specific primer 20ce: 5'-CCTCCTGAAATGTAAAACACCAAT-3'
2nd locus specific primer 20dn: 5'-CCTTCCTCTCAAATGATTGAGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11