Genotyping and primer details


Genotyping and primer details for SALK_110809c - At5g48250
Line ID SALK_110809c
Locus At5g48250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19561723 of line SALK_110809c Chr5:19561723
   
Insertion allele amplicon LBb3 - 18ck
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ck: 5'-GGAATCACATAGCTTGCATACTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19561027
   
wt amplicon (over InsPos) 21dn - 18ck
wt amplicon start Chr5:19561027
wt amplicon end Chr5:19561938
wt amplicon size 912 bp
Locus specific primer 18ck: 5'-GGAATCACATAGCTTGCATACTGA-3'
2nd locus specific primer 21dn: 5'-TCGCAAGTAAGTATAAACGCACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11