Genotyping and primer details


Genotyping and primer details for SALK_111141c - At2g34830
Line ID SALK_111141c
Locus At2g34830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14694882 of line SALK_111141c Chr2:14694882
   
Insertion allele amplicon LBb3 - 43dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43dr: 5'-TGATTAGGTTACTCGCCGTTCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14695627
   
wt amplicon (over InsPos) 05ep - 43dr
wt amplicon start Chr2:14694529
wt amplicon end Chr2:14695627
wt amplicon size 1099 bp
Locus specific primer 43dr: 5'-TGATTAGGTTACTCGCCGTTCTAT-3'
2nd locus specific primer 05ep: 5'-GCTCAATTGGATCAAGGAATAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11