Sequences from confirmation amplicon


Confirmation sequences for line SALK_111141c and hit At2g34830 are shown.
Line ID SALK_111141c
Locus At2g34830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14694882
Primers used for PCR 43dr - LBb3, show primer details
   
Sequence Name 75-K110311-SALK_111141C-43dr
GenBank Accession LN486126 [GenBank]
Sequencing primer gene specific primer, 43dr: 5'-TGATTAGGTTACTCGCCGTTCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K110311-SALK_111141C-43dr
ATATATGAACAACAAAAAAACGAGTGGAAATAGCTAGGGTTTCGTTTTGTGTAATGTATT
ATATATGTATATGAAATTTTCTGAATTCTTACATTACATTCGTCCTTTTTCTGCTGTTCA
ATTTTAATCCTGTCGTTTGCTTTTGTTAAACTTATTATTTTACTCTTTTATTTTTGCTTT
TATATGATTTTTTTTGTAGACATATTTATCTTTTCAAAACTGAAAAAGGTATGCGCAGTA
CGTAGTTATGTATGAAGTGTAAACATCATAAACAAACAATGAACATCTACATTATGCACC
ATATCTAAAACTGAACTCATCATGTCCCACACTAAAATTGACCTCAAAATCAGCTTTACA
TTACAACAGTTAGACGTATACTTACATATATATGTCTCAAACTCTCAATATACTGATGGT
TCATGCAGGAAGAGCCAGGCAAAGAAAGTGGTGTGCATACCGGCTCCAGCCGCTATGAAC
AGCCGGTCCAGTGGAGAAGTTGTTCCGTCTGATCTATGGGCTTGGCGAAAGTACGGTCAA
AAACCTATCAAAGGTTCTCCTTATCCAAGGTATATATTTACTCACATGTGTTTATATAAC
CCTATGTCTGTGACTCCGTTTCTACATATTAATTAGAAACATGAAATTTATATTGATATA
AAATATAAGTATGTTCTTTCAATAGGAATGTTTTTCTACAAATTGGAAA
   
Sequence Name 65-K110310-SALK_111141C-R210
GenBank Accession LN485868 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K110310-SALK_111141C-R210
GCGTCATTTGTTTCACCACAATTTGTTTCAATTTGTTAGAAAACATTCCTATTGAAAGAA
CATACTTATATTTTATATCTATATAAATTTCATGTTTCTAATTAATATGTAGAAACGGAG
TCACAGACATAGGGTTATATAAACACATGTGAGTAAATATATACCTTGGATAAGGAGAAC
CTTTGATAGGTTTTTGACCGTACTTTCGCCAAGCCCATAGATCAGACGGAACAACTTCTC
CACTGGACCGGCTGTTCATAGCGGCTGGAGCCGGTATGCACACCACTTTCTTTGCCTGGC
TCTTCCTGCATGAACCATCAGTATATTGAGAGTTTGAGACATATATATGTAAGTATACGT
CTAACTGTTGTAATGTAAAGCTGATTTTGAGGTCAATTTTAGTGTGGGACATGATGAGTT
CAGTTTTAGATATGGTGCATAATGTAGATGTTCATTGTTTGTTTATGATGTTTACACTTC
ATACATAACTACGTACTGCGCATACCTTTTTCAGTTTTGAAAAGATAAATATGTCTACAA
AAAAAATCATATAAAAGCAAAAATAAAAGAGTAAAATAATAAGTTTAACAAAAGCAAACG
ACAGGATTAAAATTGAACAGCAGAAAAAGGACGAATGTAATGTAAGAATTCAGAAAATTT
CATATACCTATATTATACATTACACAAAACGAAACCCTAGCTATTTCCACCTCGTTTTTT
TGTTTGTTTCAATTATTAATTTTTCAAAATATAG

Last Updated on 06.12.2018 16:11