Genotyping and primer details


Genotyping and primer details for SALK_111188c - At3g56320
Line ID SALK_111188c
Locus At3g56320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20888539 of line SALK_111188c Chr3:20888539
   
Insertion allele amplicon LBb3 - 30td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30td: 5'-TGTCCATAAAAATCGTCATCCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20888191
   
wt amplicon (over InsPos) 05ue - 30td
wt amplicon start Chr3:20888191
wt amplicon end Chr3:20888879
wt amplicon size 689 bp
Locus specific primer 30td: 5'-TGTCCATAAAAATCGTCATCCATA-3'
2nd locus specific primer 05ue: 5'-CTCACTCACTCTCTGGATTAGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11