Sequences from confirmation amplicon


Confirmation sequences for line SALK_111188c and hit At3g56320 are shown.
Line ID SALK_111188c
Locus At3g56320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20888539
Primers used for PCR 30td - LBb3, show primer details
   
Sequence Name 22-K116922-SALK_111188C-30td
GenBank Accession LN484924 [GenBank]
Sequencing primer gene specific primer, 30td: 5'-TGTCCATAAAAATCGTCATCCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116922-SALK_111188C-30td
TTTTAGTGGCACTGAACCAAATGAAAAGACCTGTAGATAAACAAGTTCCCATAGAAAGTT
AACAAAACAGCATAAATCTTTAACATGTCAGCAAAGAGAAGCGGAGTTTCTAACCTCAAT
TCCCTCATGACTCATGATAAGAGTTCGTACATAATCAATGATCTCATTCCTACTTCTGTC
TGAAACAAGGGCAGGTTGGATAGTGCAAAGTATCTCATGAGCTCTCTCCTCTGCAATCAT
CCAAGAATCTGCATCTATCGGCAATGAATCTCCTTTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAAT
AGNATGGAT
   
Sequence Name 11-K116921-SALK_111188C-R210
GenBank Accession LN484594 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116921-SALK_111188C-R210
TTTGTTTAAGGAGATTCATTGCCGATAGATGCAGATTCTTGGATGATTGCAGAGGAGAGA
GCTCATGAGATACTTTGCACTATCCAACCTGCCCTTGTTTCAGACAGAAGTAGGAATGAG
ATCATTGATTATGTACGAACTCTTATCATGAGTCATGAGGGAATTGAGGTTAGAAACTCC
GCTTCTCTTTGCTGACATGTTAAAGATTTATGCTGTTTTGTTAACTTTCTATGGGAACTT
GTTTATCTACAGGTCTTTTCATTTGGTTCAGTGCCACTAAAAACCTATCTCCCGGACGGG
GATATCGATCTGACTGTCCTAACTAAGCAAAATATGGATGACGATTTTTATGGACA

Last Updated on 06.12.2018 16:11