Genotyping and primer details


Genotyping and primer details for SALK_111230 - At3g01015
Line ID SALK_111230
Locus At3g01015 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2079 of line SALK_111230 Chr3:2079
   
Insertion allele amplicon LBb3 - 47wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47wu: 5'-TTAAACTGGGTAACCGAATACTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1577
   
wt amplicon (over InsPos) 14xf - 47wu
wt amplicon start Chr3:1577
wt amplicon end Chr3:3225
wt amplicon size 1649 bp
Locus specific primer 47wu: 5'-TTAAACTGGGTAACCGAATACTGG-3'
2nd locus specific primer 14xf: 5'-GTTTCTTCATCATGGGACAATAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11