Genotyping and primer details


Genotyping and primer details for SALK_111545c - At5g21950
Line ID SALK_111545c
Locus At5g21950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7254766 of line SALK_111545c Chr5:7254766
   
Insertion allele amplicon LBb3 - 17vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17vt: 5'-TTGTATAACTTTTAGCGTCGCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7254157
   
wt amplicon (over InsPos) 67wi - 17vt
wt amplicon start Chr5:7254157
wt amplicon end Chr5:7255631
wt amplicon size 1475 bp
Locus specific primer 17vt: 5'-TTGTATAACTTTTAGCGTCGCCTT-3'
2nd locus specific primer 67wi: 5'-CTCACTTCTTTCGTCTCTACGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11