Genotyping and primer details


Genotyping and primer details for SALK_112126c - At3g49560
Line ID SALK_112126c
Locus At3g49560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18371510 of line SALK_112126c Chr3:18371510
   
Insertion allele amplicon LBb3 - 28ie
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28ie: 5'-TATAACCCAATGGGAGATGAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18370535
   
wt amplicon (over InsPos) 04jw - 28ie
wt amplicon start Chr3:18370535
wt amplicon end Chr3:18371781
wt amplicon size 1247 bp
Locus specific primer 28ie: 5'-TATAACCCAATGGGAGATGAGAAG-3'
2nd locus specific primer 04jw: 5'-AAAGAACAGAGAATAGCTCCCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11